Table 3.
Comparison of fermentation performance of improved strains with mutated LpXI
| Strain | LpXI | Cxyl36 (g/L/h)a | Cxyl72 (g/L/h)b | Yeth72 (g/g-input sugars)c |
|---|---|---|---|---|
| SS81 | Wild-type | 0.36 ± 0.08 | 0.30 ± 0.08 | 0.39 ± 0.03 |
| SS82 | T63I/A121A | 0.68 ± 0.06 | 0.47 ± 0.01 | 0.44 ± 0.01 |
| SS92 | V162A/T273T/N303T | 0.76 ± 0.04 | 0.48 ± 0.02 | 0.44 ± 0.01 |
| SS104 | V162A | 0.59 ± 0.05 | 0.46 ± 0.01 | 0.43 ± 0.01 |
| SS105 | N303T | 0.32 ± 0.06 | 0.25 ± 0.02 | 0.40 ± 0.01 |
| SS120 | T63I/V162A | 0.75 ± 0.05 | 0.49 ± 0.00 | 0.44 ± 0.01 |
aCxyl36: d-xylose consumption rate in 36 h
bCxyl72: d-xylose consumption rate in 72 h
cYeth72: ethanol yield in 72 h per input sugars