FIG. 5.
Phosphorylation of GluR1 and GR in the CeA 2 weeks post-TBI. A subset of animals was sacrificed after behavioral testing (2 weeks post-TBI) for western blot analysis of phosphorylated GluR1 at Ser845 (pGluR1S845) and phosphorylated GR at Ser232 (pGRS232) in CeA tissue of sham-vehicle (open bars), TBI-vehicle (black bars), and TBI-JZL184 animals (gray bars). (A) Ratio of pGluR1S845 to total GluR1 in the CeA. (B) Ratio of pGRS232 to total GR in the CeA. *p < 0.05 compared with sham-vehicle group. #p < 0.05 compared with TBI-vehicle group. Data in all panels analyzed by one-way ANOVA with Tukey's multiple comparisons test, n = 4–6/group. Data in all panels are expressed as mean ± SEM. ANOVA, analysis of variance; CeA, central amygdala; GluR1, glutamate receptor subunit 1; GR, glucocorticoid receptor; SEM, standard error of the mean; TBI, traumatic brain injury.