Table 3.
Ratio of strong positive pixels (strong staining) on total pixels and weak positive pixels (weak staining) on total pixels for GFAP for the 6 different regions of interest and for all regions.
| GFAP |
||||||||
|---|---|---|---|---|---|---|---|---|
| Sham |
TBI |
HH |
TBI+HH |
|||||
| Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | |
| Neocortex | 36.9 ± 2.1 | 16.4 ± 3.1 | 24.3 ± 1.1* | 28.7 ± 2.3 | 17 ± 0.7*§ | 32.9 ± 1.6* | 17.1 ± 1*§ | 32.4 ± 2.1* |
| Striatum | 28.4 ± 1.8 | 19.7 ± 3.1 | 18.5 ± 2.3* | 35.1 ± 3.5* | 17.7 ± 1.2* | 34.5 ± 2.2* | 20.4 ± 1.8* | 31.4 ± 3* |
| Thalamus | 27.7 ± 1.3 | 19.9 ± 3.2 | 20.1 ± 1.5 | 25.4 ± 3.1 | 20.9 ± 1.6 | 27.1 ± 2.3 | 20.8 ± 1.8 | 25.3 ± 2.6 |
| Dentate gyrus | 38.7 ± 3.4 | 10.6 ± 2.4 | 29 ± 2 | 18.1 ± 2.4 | 30.8 ± 2.1 | 18.1 ± 2.3 | 29.4 ± 3.6 | 21.2 ± 3.6 |
| CA-1 region | 35.7 ± 3.1 | 12.3 ± 2.3 | 29.7 ± 2.3 | 16.4 ± 2.4 | 28.8 ± 2.1 | 18.4 ± 2.5 | 29.7 ± 3.1 | 17.9 ± 3.3 |
| Corpus callosum | 37.2 ± 4.9 | 11 ± 3 | 33 ± 4.7 | 17.6 ± 3.6 | 27.7 ± 1.6 | 17.1 ± 2.1 | 28.8 ± 1.6 | 14.9 ± 1.9 |
| All regions | 34 ± 1.3 | 15.1 ± 1.3 | 25.5 ± 1.2* | 23.8 ± 1.5* | 21.8 ± 0.7*§ | 27.1 ± 1.1* | 22.5 ± 0.9* | 26 ± 1.3* |
Abbreviations: GFAP, glial fibrillary acidic protein; HH, hypoxia-hypotension; SEM, standard error of the mean; TBI, traumatic brain injury.
Ratios are expressed in percentage of total pixels. Data are expressed in mean ± SEM.
*
P < .05 vs Sham group. §P < .05 vs TBI group.