Table 4.
Ratio of strong positive pixels (strong staining) on total pixels and weak positive pixels (weak staining) on total pixels for UCH-L1 for the 6 different regions of interest and for all regions.
| UCH-L1 |
||||||||
|---|---|---|---|---|---|---|---|---|
| Sham |
TBI |
HH |
TBI+HH |
|||||
| Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | Strong staining (%) | Weak staining (%) | |
| Neocortex | 6.4 ± 1.3 | 17.7 ± 4.1 | 11.1 ± 2.1* | 9.6 ± 3* | 13.1 ± 1* | 5.2 ± 0.7* | 13.2 ± 1.2* | 6.6 ± 1* |
| Striatum | 5.5 ± 1.4 | 23.4 ± 8.9 | 5 ± 1.3 | 18.7 ± 6.1 | 16.6 ± 2.4*§ | 4.7 ± 1.6*§ | 14.9 ± 2.3*§ | 7.1 ± 2.1*§ |
| Thalamus | 9.3 ± 5.9 | 21.7 ± 7 | 16.1 ± 5.8 | 12.9 ± 5.9 | 24.4 ± 2.3 | 4.1 ± 0.8 | 19.6 ± 2.7 | 6.4 ± 1.2 |
| Dentate gyrus | 5 ± 1.5 | 20.3 ± 6.5 | 6.4 ± 2.3 | 19.3 ± 4.7 | 14.7 ± 2.1*§ | 4.5 ± 0.8*§ | 12.6 ± 2.1* | 6.6 ± 2.5*§ |
| CA-1 region | 7.5 ± 1.8 | 18 ± 4.7 | 7.3 ± 1.9 | 12.9 ± 3.1 | 18.4 ± 1.8*§ | 3.1 ± 0.5*§ | 13.1 ± 2.7 | 5.5 ± 1.7* |
| Corpus callosum | 8.4 ± 2.6 | 14.6 ± 3.5 | 6.9 ± 1.3 | 13.5 ± 5.7 | 17.1 ± 1.8*§ | 5.1 ± 1.1*§ | 18.2 ± 4.2*§ | 5.8 ± 1.3* |
| All regions | 7 ± 0.9 | 18.2 ± 2.2 | 9.5 ± 1.2 | 13.1 ± 1.8* | 15.7 ± 0.7*§ | 4.7 ± 0.4*§ | 14.3 ± 0.9*§ | 6.4 ± 0.6*§ |
Abbreviations: HH, hypoxia-hypotension; SEM, standard error of the mean; TBI, traumatic brain injury; UCH-L1, ubiquitin carboxy-terminal hydrolase-L1.
Ratios are expressed in percentage of total pixels. Data are expressed in mean ± SEM.
*
P < .05 vs Sham group. §P < .05 vs TBI group.