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. 2019 Apr 9;294(22):8717–8731. doi: 10.1074/jbc.RA118.006423

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© 2019 Mathiesen et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Confirmation of syndecan-4 binding of selected putative partners in HEK293. HEK293 cells were co-transfected with HA-tagged syndecan-4 and 3×FLAG–MPP7 (A); 3×FLAG–PARVB (B); 3×FLAG–CDK9 (C); 3×FLAG–EIF2S1 (D); PFKM–FLAG (E); CCT5–FLAG (F); RASIP1–FLAG (G); EIF4B–FLAG (H); PTRF–FLAG (I); 3×FLAG–ADD3 (negative control) (J); or 3×FLAG–STK39 (negative control) (K). HEK293 lysates were subjected to immunoprecipitation using anti-FLAG. Immunoprecipitates (two upper most panels) and lysates (two lower most panels) were analyzed by immunoblotting with anti-HA or anti-FLAG. Expression of RASIP1 was too low to be detected without IP. The 25-kDa core syndecan-4 protein (arrow) interacted with PTRF-FLAG in I.