Figure 6.

Knockdown of TXNDC5 in lung cancer cells leads to more localization of Srx in the cytosol. A, two shRNAs targeting different coding regions of TXNDC5 were used to establish stable knockdown in A549 cells. Knockdown of TXNDC5 does not affect the endogenous expression of Srx or TXNDC7, a close member of TXNDC5 in the PDI family. B, subcellular fractionation of A549 control (ShNT) or TXNDC5 knockdown cells for the distribution of Srx in ER and cytosol. Results from three independent replicates were shown. The bar graph with a dot plot on the right indicates the quantitative results (*, p < 0.05, ANOVA). Error bars, S.D.