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. 2019 Apr 18;294(22):8872–8884. doi: 10.1074/jbc.RA118.007040

Figure 6.

Figure 6.

RIPK3 mediates the NLRP3 inflammasome-dependent IL-1β cleavage and release in response to HMGB1 and microbial lipids. A, IL-1α and IL-1β measured from culture supernatants of peritoneal macrophages from WT, Ripk3−/−, Nlrp3−/−, and Asc−/− mice stimulated with lipid IVa or lipid A (1 μg/ml) in the absence or presence of HMGB1 (0.4 μg/ml). B and C, Western blot analysis of IL-1α and IL-1β released from mouse peritoneal macrophages with the indicated genotypes stimulated with lipid IVa (1 μg/ml) + HMGB1 (0.4 μg/ml) (B) or lipid A (1 μg/ml) + HMGB1 (0.4 μg/ml) (C). D, Western blot analysis of processed IL-1α and IL-1β released from mouse peritoneal macrophages with the indicated genotypes stimulated with lipid IVa (1 μg/ml) + HMGB1 (0.4 μg/ml) or lipid A (1 μg/ml) + HMGB1 (0.4 μg/ml). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; #, not significant. Graphs show the mean ± S.D. from three independent experiments.