Figure 4.

Interaction between cohesin and splicing factors occurs in chromatin and does not require DNA or RNA. A, dual-affinity purifications from RNase- and DNase-treated nuclear extracts of HCT116 cells and SMC3 epitope-tagged derivatives were separated by SDS-PAGE, and Western blotting was performed with the antibodies indicated. B, fractionated chromatin and soluble lysates were prepared from HeLa cells, immunoprecipitated (IP) with SMC1A antibodies or IgG control antibodies, and separated by SDS-PAGE, and Western blotting was performed with the antibodies indicated.