Figure 2.

ER stress inducers that do not directly target Ca²⁺ stores do not trigger NOD-dependent pro-inflammatory cytokine expression. A and B, expression of HSPA5, CXCL1, and IL8 in WT, NOD1 and NOD2 KO, and NOD1/2 DKO HCT116 cells following stimulation with 1 μg/ml tunicamycin (Tunic) (A) or 50 ng/ml SubAB toxin from Shiga toxigenic E. coli, either WT (sAB) or mutated on position 272 (sA272B), which abolishes its activity, for 4 h (B) and measured by qRT-PCR. In all data sets, each point is from an independent experiment and is the average of three technical replicates. *, **, and ****, represents p < 0.05, p < 0.01, and p < 0.0001, respectively. ns, not significant; Ctl, control.