Figure 2.

Lack of ACSL1 decreased muscle glycogen content, but glucose use was unchanged. A, TAG content of white gastrocnemius (WGastroc), red gastrocnemius (RGastroc), and soleus, n = 3–4 per genotype. B, glycogen content of white gastrocnemius, red gastrocnemius, and soleus in unfasted mice at 10 a.m. to 11 a.m., n = 7–9 per genotype. C, 2-[1-¹⁴C]deoxyglucose uptake in gastrocnemius in mice deprived of food for 3 h and then exposed to 4 °C for 2 h, n = 3 per genotype. D, [2-¹⁴C]pyruvate oxidation in white and red gastrocnemius homogenates, n = 4–5 per genotype. E–J, hyperinsulinemic-hyperglycemic clamp study, n = 8–9 per genotype; E, glucose was clamped at 225 mg/dl; F, glucose concentrations at 0 and 140 min (clamped). G, insulin concentration at 0 and 140 min (clamped); H, whole-body glucose uptake. I, glucose infusion rate. J, area under curve for glucose infusion rate. Data are presented as means ± S.D. (error bars). **, p < 0.01; *, p < 0.05.