Figure 5.

Deletion of Acsl1 resulted in skeletal muscle cell apoptosis. A, mice were deprived of food for 4 h, muscles were excised, and muscle homogenates were subjected to Western blot analysis and immunoprobed with anti-caspase-3 and anti-GAPDH. B, gastrocnemius was fixed in 10% formalin and immunostained with anti-cleaved caspase-3. Shown are representative 50-μm images of immunostained cleaved caspase-3. C, representative images of gastrocnemius immunostained with anti-F4/80, a macrophage cell surface marker.