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. 2019 Jun 3;7:e7023. doi: 10.7717/peerj.7023

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© 2019 Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Flow cytometric analysis of ex-vivo-expanded hUCMSCs, hBMSCs, and hSHED. All the three MSC populations revealed positive expression of CD105, CD90, CD73, and negative expression of CD45, CD34, CD14, CD19, and HLA-DR. (B) After osteogenic induction for 28 days, hUCMSCs, hBMSCs, and hSHED formed mineralized nodules stained by Alizarin Red. (C) After culturing in adipogenesis inducing medium for 14 days, hUCMSCs, hBMSCs, and hSHED were found to form lipid droplets stained with Oil Red O.