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. 2019 Jun 3;7:e7023. doi: 10.7717/peerj.7023

Figure 5. MSCs reduced inflammatory cytokine expression in serum and hind limb ankle joins.

Figure 5

Level of serum total TNF-α (A) and IL-1β (B) was measured by ELISA. MSC treatment reduced expression of TNF-α and IL-1β. The expression of TNF-α and IL-1β decreased in UC group compared to BMSC group and SHED group, while the expression was lower in the BMSC group compared to the SHED group. Representative images of immunoreactivity for TNF-α (C and D) and IL-1β (E and F) in hind limb ankle joins of the mice. The expressions of TNF-α and IL-1β decreased in MSC treatment groups compared with CIA group. UC group showed lower TNF-α and IL-1β compared to the BMSC group or the SHED group, while the expression was lower in the BMSC group compared to the SHED group. The histological score of each group was evaluated and presented in column. Scale bar, 75 μm. The data indicate the mean ± SD of six mice per group from three independent experiments. The data were analyzed using one-way ANOVA for comparison among multiple groups and Tukey’s multiple comparisons test for comparison between two groups (*P < 0.05, **P < 0.01, ***P < 0.001).