Table 1. Sequencing methods & sample procurement information.
| Species | Single/ paired end | Library prep. method | Voucher/PI/MSB/W6 |
|---|---|---|---|
| Amelichloa brachychaeta | Single | Nextera XT | PI 197978 |
| Ammochloa palaestina | Paired | Nextera | R. Lazaro, s.n. (US) |
| Arrhenatherum elatius | Single | Nextera XT | PI 665562 |
| Castellia tuberculosa | Single | Nextera XT | PI 238257 |
| Catapodium marinum | Single | Nextera XT | MSB 53132 |
| Desmazeria sicula | Paired | Nextera | MSB 17332 |
| Dichelachne crinita | Single | Nextera XT | W6 22474 |
| Festuca ovina | Paired | Nextera | PI 655206 |
| Kengyilia melanthera | Single | Nextera XT | PI 639856 |
| Koeleria nitidula | Single | Nextera XT | PI 206688 |
| Nephelochloa orientalis | Single | Nextera XT | W6 19223 |
| Poa subgen. Stenopoaa | Paired | Nextera | PI 374046 |
| Polypogon fugax | Single | Nextera XT | PI 220619 |
| Scolochloa festucacea | Paired | Nextera | Thompson, 866 (ISC) |
| Triplachne nitens | Single | Nextera XT | MSB 26060 |
| Ventenata macra | Single | Nextera XT | PI 204431 |
Notes.
T1. Sequencing methods for species included in this study. Procurement information for species sequenced for this study either as seed accessions or as herbarium vouchers. Seed accessions procured from: USDA Plant Introduction (PI), West Regional PI Group (W6), MSB (Kew Millenium Seed Bank).
a
Poa subgen. Stenopoa was originally misidentified as Festuca pseudovina (USDA, 2017a). After DNA barcoding of ETS and ITS nuclear genes, additional plastid genes/IGS (trnT-trnL-trnF, matK, trnC-rpoB) were also examined, as well as a morphological assessment; it was determined that this grass is identified as a member of the subgenus Stenopoa, however no specific determination could be made as to the exact species.