Fig. 5.

IMS enabled the detection of specific GM3 ganglioside isoforms in a human pancreas. Immunostaining (green, C-peptide; red, glucagon; blue, DAPI) and false-colour 30 μm spatial resolution IMS from serial tissue sections showing the spatial distribution of a series of GM3 ganglioside ions in human pancreatic tissue from a 20-year-old donor. Lipids were identified by accurate mass measurements (ppm error reported). Ion images were normalised to TIC and are shown with pixel interpolation as ±0.05 Da. Magnifications of the area highlighted with white dotted lines in the IHC image are shown below each IHC and IMS image. Intensity scales of the false-colour IMS images are shown in the bottom right of each magnified image. All scale bars are 100 μm. C-PEP, C-peptide; GCG, glucagon