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. Author manuscript; available in PMC: 2019 Jun 6.
Published in final edited form as: Mol Nutr Food Res. 2018 Jul 19;62(18):e1800228. doi: 10.1002/mnfr.201800228

Figure 1.

Figure 1.

Decreased HDAC3 expression and increased pH2AX in the Pirc model. Tumor and adjacent normal colon from Pirc males (≈8 months old) were probed for HDAC3 and pH2AX by A) immunoblotting with β-actin as loading control and B) immunohistochemistry (IHC); T, tumor; N, normal; white arrows, pH2AX immunostaining. C) Pirc males received corn oil vehicle or ITC (60 mg kg–1) by single oral gavage and were sacrificed 6 h later. Tumor tissue lysates (n = 3) were immunoblotted for pH2AX, H2AX, HDAC3, and SIRT6, with β-actin as loading control. D–F) Immunoblots were quantified by densitometry and the data plotted as mean ± SEM (n = 3) by one-way ANOVA (**p < 0.01, compared to vehicle). G) Immunostaining of pH2AX in Pirc colon polyps after IHC analyses.