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Cytotoxicity of SFN is dependent on plating density. HCT116 colon cancer cells were seeded at two different cell densities (30%, sub-confluent; 80%, confluent) and treated for 24 h with SFN in the dose range 0–60 μm. Cell viability was assessed using the CCK-8 assay (n = 4 experiments); *p < 0.05, < 0.01, confluent versus the corresponding sub-confluent cell density data point using Student’s t-test.
