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. Author manuscript; available in PMC: 2020 Mar 19.
Published in final edited form as: Immunity. 2019 Feb 26;50(3):707–722.e6. doi: 10.1016/j.immuni.2019.02.002

Figure 2: ILC2s are associated with CD11b+ dendritic cells at cuff sites.

Figure 2:

(A-C) 3D rendering with zoom of 200μm projections from (A) lung, (B) liver, or (C) kidney, highlighting IL-5 RFP+ ILC2 and CD11c+ MHCII+ myeloid cells, most consistent with dendritic cells (DCs). (D) Image analysis of 3D thick sections from lung, liver, and kidney for indicated populations; association defined as <900nm between the nearest ILC2 and DC surface vertices. (E) Flow cytometry analysis of lung DC subsets. (F-I) 2D-thin cut images of ILC2 localization (yellow arrows) with (F) CD11b+ DCs or (G and H) CD301b+ CD11b+ DCs from indicated tissues, with (I) quantification of co-localization. Occasional CD11b-neg DCs (F) or CD301b-neg DCs (G) are indicated with stars. (A-C, E-H) Representative of three or more mice. (D,I) Data points represent averages of individual mice n=3–4 with >n=25 cells analyzed for per animal. See also Figure S3.