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. 2019 Apr 4;39(6):1055–1071. doi: 10.1161/ATVBAHA.119.312399

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© 2019 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 4. — Pseudotime analysis discovers a proinflammatory role of Mesen II cluster. A, Distinct states of cells identified by pseudotime analysis. B, Ordering of cells from different nonimmune clusters along the pseudotime trajectory. C Heatmap of the significantly changed genes (P<0.01) discovered by the BEAM function from monocle in branch point 2. D, Expression level of Ly6a and Cd248 along the pseudotime trajectory. E, Heatmap showing the expression level of significantly changed genes (P<0.01) in the gene ontology (GO) term cytokine activity. No significantly changed genes from cytokine activity GO term was found in gene blocks 2 and 3. F, Expression level of Ccl2 and Ccl11 along the pseudotime trajectory. Adv-EC indicates adventitial endothelial cell; BEAM, branch expression analysis modeling; and SMC, smooth muscle cell.