Figure 1.

Identification of 6mA positions in the Drosophila melanogaster genome. Panel A: Flowchart showing the filtering steps involved in identifying the high-confidence 6mA positions. Panel B: Boxplot showing the difference in signal level between the PCR-free and control sequencing libraries for the high-confidence 6mA positions and unmethylated positions. As expected, the adenine positions that were identified as methylated show a significantly larger difference in signal level compared to the unmethylated positions (Wilcoxon Test P < 2.2e-16).