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. 2019 Apr 16;9(6):1933–1943. doi: 10.1534/g3.119.400162

Figure 2.

Figure 2

Insertion of GFP11 tag has mild or no effect on egg numbers and viability. P0 were scored for number of F1 eggs and adults. A and B) egg numbers: each data point is egg number of a single P0. C and D) F1 numbers: each data point is F1 adult number of a single P0. E and F) Viability, each data point is percent of F1 divided by egg numbers. Values that are not significant are not shown as well as 3 pairwise comparisons in which the mutant had greater viability: ollas::rpa-1 had more F1s/P0 compared to wild-type (P = 0.0447), gfp1-10; gfp11::rpa-1 had higher viability compared to gfp1-10 and gfp1-10; gfp11::rpa-1 (P = 0.0173). Statistics: Kruskal-Wallis test was performed on each panel (egg, adult and viability) and when significant Mann-Whitney U-test was performed on every pairwise combination to identify which combinations show reduced viability compared to wild-type (A, C and E) and gfp1-10 strain (B, D and F).