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. 2019 Apr 16;9(6):1933–1943. doi: 10.1534/g3.119.400162

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Copyright © 2019 Hefel, Smolikove

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The split sfGFP approach is suitable for live imaging Expression of GFP11 tagged proteins in the germline expressing GFP1-10 strain leads to localization pattern visible by live imaging. Top is DIC channel and GFP channel, bottom are images taken from the same region just with GFP. Green- live imaging GFP channel. Stages indicated above include the mitotic pre-meiotic tip, and the meiotic stages of mid pachytene, late pachytene, diakinesis (-3 oocyte) and diakinesis (-1 oocyte). Regions marked with * are gut regions that show autofluorescence. All images are slices of 0.8μm close to the mid-section of the nuclei, except SYP-4 which is projection throughout the nuclei. Scale bars 2μm.