Figure 6.

Increasing the GFP11 tag number to three slightly improves fluorescence intensity of GFP11-AKIR-1 A) Expression of 3XGFP11 tagged AKIR-1 in the germline expressing GFP1-10. Blue- DAPI, Green- GFP. Stages indicated above include the mitotic pre-meiotic tip, and the meiotic stages of diakinesis (-1 oocyte). Scale bars 2μm. B) Nuclear intensity calculated as Raw intensity/Area(µm2), for diakinesis (-1 oocyte). C) Nuclear intensity normalized: nuclear Raw intensity/Area(µm²) from which cytoplasmic background [Raw intensity/Area(µm²)] was subtracted.