Table 1. Sequences used for CRISPR/Cas9, MosSCI, cloning and diagnosis. Table of the DNA/RNA sequences used for construction of the strains in this manuscript.
| Sequence name | Sequence |
|---|---|
| ollas::rpa-1 ssODN | Ttccccaatttttatgtatctgtttcagatagtgaaagatgtccggattcgccaacgagctcggaccacgtctcatgggaaaggcggcaattcacatcaatcacgatgtcttcaataa |
| gfp11::rpa-1 ssODN | ttccccaatttttatgtatctgtttcagatagtgaaagatgCGTGACCACATGGTCCTCCACGAGTACGTCAACGCCGCCGGAATCACCGGTGGCGGCAAATTCgcggcaattcacatcaatcacgatgtcttcaataa |
| gfp11::akir-1 ssODN | ttacttctcgtaaccacaaattatttctttcagaagtaaaatgCGTGACCACATGGTCCTCCACGAGTACGTCAACGCCGCCGGAATCACCGGTGGCGGCAAATTCGCTTGCGGACTCGCACTGAAAAGACCTCTCCAACATGAGTACGAGTCTTTTTTAACTGATGAGACATACAACGGAGAAGCAAAGCGAGCC |
| gfp11::syp-4 ssODN | gttcggtacggtaacctcatttttcatcaaaattttttatttcaaggcgaaataatgCGTGACCACATGGTCCTCCACGAGTACGTCAACGCCGCCGGAATCACCGGTGGCGGCAAATTCtcgtttccgacgTtacaagtGAgAccaaatgagaaaaatccaaaagttctgcgatgcc |
| 3xgfp11 ssODN | ATGCGTGACCACATGGTCCTCCACGAGTACGTCAACGCAGCCGGAATCACCGGTGGCGGCAAATTCCGTGACCACATGGTCCTCCACGAGTACGTAAACGCAGCCGGAATCACCGGTGGCGGCAAATTCCGTGACCACATGGTCCTCCACGAGTACGTAAACGCCGCCGGAATCACCGGTGGCGGCAAATTC |
| rpa-1 crRNA | UUUCAGAUAGUGAAAGAUGG |
| akir-1 crRNA | GAUUCAUACUCGUGUUGCAG |
| syp-4 crRNA | UUUGGACGUACUUGUAGCGU |
| gfp11 crRNA | CACGAGUACGUCAACGCCGC |
| gfp1-10 g-block with introns | ATGAGTAAAGGAGAAGAATTGTTCACTGGAGTTGTCCCAATCCTCGTCGAGCTCGACGGAGACGTCAACGGACACAAGTTCTCCGTCAGAGGAGAGGGAGAGGGAGACGCCACCATCGGAAAGCTCACCCTCAAGTTCATCTGCACCACCGGAAAGCTCCCAGTCCCATGGCCAACCCTCGTCACCACCTTGACATACGGAGTCCAATGCTTCTCCCGTTACCCAGACCACATGAAGCGTCACGACTTCTTCAAGTCCGCCATGCCAGAGGGATACGTCCAAGAGCGTACCATCTCATTCAAGGTAAGTTTAAACATATATATACTAACTACTGATTATTTAAATTTTCAGGACGACGGAAAATACAAGACCCGTGCCGTGGTCAAGTTCGAGGGAGACACCCTCGTCAACCGTATCGAGCTCAAGGTAAGTTTAAACAGTTCGGTACTAACTAACCATACATATTTAAATTTTCAGGGAACTGACTTCAAGGAGGACGGAAACATCCTCGGACACAAGCTCGAGTACAACTACAACTCCCACAACGTCTACATCACAGCCGACAAGCAAAAGAACGGAATCAAGGCCAACTTCACTATCCGTCACAACATCGAGGACGGATCCGTCCAACTCGCCGACCACTACCAACAAAACACCCCAATCGGAGACGGACCAGTCCTCCTCCCAGACAACCACTACCTCTCCACCCAAACCGTCCTCTCCAAGGACCCAAACGAGAAG |
| gfp1-10 F | CAAGAGCGTACCATCTCATTCAAG |
| gfp1-10 R | GTTGTGGGAGTTGTAGTTGTACTC |