FIG 1.

Phylogenetic analysis of the ITS regions shows that the 16 R. mucilaginosa strains form a clade separate from other Rhodotorula spp. and from other basidiomycetes. The evolutionary history was inferred by using the maximum likelihood method based on the Kimura 2-parameter model (37). The tree with the highest log likelihood value (−4,170.28) is shown. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 25 nucleotide sequences. All positions with less than 95% site coverage were eliminated. There were a total of 785 positions in the final data set. Evolutionary analyses were conducted in MEGA7 (38). R. kratochvilovae, Rhodotorula kratochvilovae; R. sphaerocarpa, Rhodotorula sphaerocarpa; A. fumigatus, Aspergillus fumigatus; H. capsulatum, Histoplasma capsulatum; B. gilchristii, Blastomyces gilchristii.