Fig 2. Integrity of mtDNA is unaffected upon loss of mitochondrial fusion.

Analysis of mitochondrial DNA rearrangement frequency from hearts of control (A), dMfn KO (B), and Deletor (C) animals at 5 weeks of age as determined by Illumina sequencing (control n = 3; dMfn KO n = 3 and Deletor mouse n = 1). The graphs depict paired-end sequencing reads from individual animals with an abnormally long or short insert size plotted as bins along the x-axis. The frequency of unique rearrangement breakpoints is plotted on the y-axis. Blue bins represent variations in the fragment size and not rearrangement breakpoints; red bins denote rearrangements larger than 600 bases. In the deletor mice, a green bin at position 16,400 kb signifies rearrangement breakpoints encompassing the first and last positions of the reference sequence, leading to difficulties in assessing the true size of the rearrangement. Shades of blue and red indicate different individual animals within a group. (D) Mitochondrial DNA mutation load analysis in heart from control and dMfn KO animals at 5–6 weeks of age, n = 3 for both genotypes. n.s., no statistical significance based on Student T-test.