Fig 5. 2DG treatment at day 3 did not affect suppressive capability of Treg subsets.

Suppression assays were performed with 7 days cultured tTreg (red) and iTreg (blue) after they were rested in 50IU/ml IL-2 medium for an additional 2 days prior to assay. Autologous CD4 enriched T responders were stimulated by CD3/CD28 microbeads at 1:5 bead to cell ratio for 84hrs in absence or presence of suppressors indicated effector to suppressor ratio. Representative histogram gated on live CD4+ responder show the CFSE dilution profiles with stimulation control (open black) and unstimulated control (filled grey). % inhibition of CD4+ T cell proliferation was calculated as %proliferating (stimulated-sample)/ %proliferating cells (stimulated—unstimulated). Representative data is shown from 3 independent experiments of total 6 individual donors with experimental duplicates.