Table 1. Hemolysis inhibitors.
Treatment | CST SRBC Hemolysis (% max) | AST SRBC Hemolysis (%max) |
---|---|---|
Turtle plasma | 94.8 ± 2.3 | 81.4 ± 0.8 |
Turtle plasma (56°C, 30 min) | 2.2 ± 0.1 | 1.3 ± 0.1 |
Turtle plasma + 100 U protease | 0.9 ± 0.1 | 0.4 ± 0 |
Plasma + 5 mM EDTA | 4.1 ± 0.3 | 2.2 ± 0.3 |
Plasma + 20 mM phosphate | 5.0 ± 0.3 | 2.7 ± 0.3 |
Plasma + 20 mM citrate | 4.3 ± 0.1 | 2.7 ± 0.1 |
Plasma + 20 mM ammonia | 93.1 ± 0.7 | 83.4 ± 1.5 |
Plasma + 20 mM methylamine | 93.3 ± 1.1 | 83.0 ± 1.2 |
Plasma + 50 mM salicylaldoxime | 9.8 ± 0.4 | 34.7 ± 0.5 |
Effects of mild heat treatment, protease, and chemical inhibitors on SRBC hemolysis by plasma from Common (Chelydra serpentina; CST) and Alligator (Macrocheyls temminckii; AST) snapping turtles