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. 2019 Jun 6;14(6):e0217626. doi: 10.1371/journal.pone.0217626

Table 1. Hemolysis inhibitors.

Treatment CST SRBC Hemolysis (% max) AST SRBC Hemolysis (%max)
Turtle plasma 94.8 ± 2.3 81.4 ± 0.8
Turtle plasma (56°C, 30 min) 2.2 ± 0.1 1.3 ± 0.1
Turtle plasma + 100 U protease 0.9 ± 0.1 0.4 ± 0
Plasma + 5 mM EDTA 4.1 ± 0.3 2.2 ± 0.3
Plasma + 20 mM phosphate 5.0 ± 0.3 2.7 ± 0.3
Plasma + 20 mM citrate 4.3 ± 0.1 2.7 ± 0.1
Plasma + 20 mM ammonia 93.1 ± 0.7 83.4 ± 1.5
Plasma + 20 mM methylamine 93.3 ± 1.1 83.0 ± 1.2
Plasma + 50 mM salicylaldoxime 9.8 ± 0.4 34.7 ± 0.5

Effects of mild heat treatment, protease, and chemical inhibitors on SRBC hemolysis by plasma from Common (Chelydra serpentina; CST) and Alligator (Macrocheyls temminckii; AST) snapping turtles