Table 1. Oligonucleotide primers used for the amplification of ama1 gene fragments.

Gene fragment	Primer name	Primer sequence	Corresponding protein residues
ama1	AMA1NC-For AMA1NC-Rev AMA1NN-For AMA1NN-Rev AMA1C-For AMA1C-Rev AMA1NN-For AMA1C-Rev	5’-TGGATCGCAAGCGATCACGTCGGGGAATCCCTTTC-3’ 5’-CAAGCTTGTCGACGGTTGGGCATTTACTGATGAACGCATC TGGG-3’ 5’-TGGACAGCCCAGATCCGTCGGGGAATCCCTTTCAG-3’ 5’-CGCTGGCGTCAGATCTGGGCATTTACTGATGAACGCATCT-3’ 5’-TGGACAGCCCAGATCCAAATCAAGCTCTTCGCGGGTACAG-3’ 5’-CGCTGGCGTCAGATCGTAATCCCCCTCGACCATAACATGT-3’ 5’-TGGACAGCCCAGATCCGTCGGGGAATCCCTTTCAG-3’ 5’-CGCTGGCGTCAGATCGTAATCCCCCTCGACCATAACATGT-3’	AMA1-N 67–287 (221 aa) (add after ROP1) AMA1-N 68–287 (220 aa) (add before SAG2) AMA1-C 287–569 (283 aa) (add before SAG2) AMA1 68–569 (502 aa) (add before SAG2)