Table 2. Plasmid size, amino acid composition and characteristics of the recombinant chimeric tetravalent proteins.

Plasmid (size)	Amino acid residues	Protein characteristic
pET30/SAG2-GRA1-ROP1-AMA1N (7890 bp)	31–170 SAG2 26–190 GRA1 85–396 ROP1 67–287 AMA1N	892 aa Mw 96.96 kDa pI 5.62
pET30/AMA1N-SAG2-GRA1-ROP1 (7911 bp)	68–287 AMA1N 31–170 SAG2 26–190 GRA1 85–396 ROP1	899 aa Mw 97.95 kDa pI 5.54
pET30/AMA1C-SAG2-GRA1-ROP1 (8097 bp)	287–569 AMA1C 31–170 SAG2 26–190 GRA1 85–396 ROP1	961 aa Mw 103.20 kDa pI 5.00
pET30/AMA1-SAG2-GRA1-ROP1 (8757 bp)	68–569 AMA1 31–170 SAG2 26–190 GRA1 85–396 ROP1	1182 aa Mw 128.42 kDa pI 5.26