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. 2019 Jun 6;14(6):e0217897. doi: 10.1371/journal.pone.0217897

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© 2019 Hayama et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Chemical conjugation of Nsp1FG to a silica surface. (B) Representative sensorgram of Nsp1FG conjugation. Changes in resonance frequency (ΔF₅) (bottom, blue) and energy dissipation (ΔD₅) (top, red) for the 5^th overtone recorded in real time. Nsp1FG conjugation step, wash, passivation with mPEG, and beta-mercaptoethanol treatment are indicated. (C) Viscoelastic modeling of the Nsp1FG surface at different density estimates (top: viscosity, middle: shear modulus, bottom: layer thickness).