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. 2019 Jun 6;14(6):e0218003. doi: 10.1371/journal.pone.0218003

Fig 1. PINK1 deficient lung epithelial cells selectively release mitochondrial DNA.

Fig 1

(A) PINK1 mRNA levels and mtDNA copies in culture media in A549 cells treated with increasing doses of TM for 24h (n = 3; *p<0.01 vs TM 0μg/ml; two-way ANOVA with multiple comparison). (B) Mitochondrial and nuclear DNA copies detected in culture media in A549 cells treated with TM 1μg/ml for 24h (n = 4; **p<0.0001; two-way ANOVA with multiple comparison). (C) PINK1 mRNA levels in primary human alveolar epithelial cells treated 24h with TM 0.1μg/ml (n = 4; **p<0.0001; unpaired t-test). Mitochondrial and nuclear DNA copies detected in culture media in primary human alveolar epithelial cells treated 24h with TM 0.1μg/ml. (n = 4; **p<0.0001; two-way ANOVA with multiple comparison). (D) MtDNA copies in culture media in PINK1 knock-down A549 cells (n = 3, *p<0.001 vs scramble; unpaired t-test). (E) MtDNA copies detected in the BAL fluid of PINK1 +/+ and -/- mice (n = 4, *p<0.001 vs PINK1 +/+; unpaired t-test). (F) MtDNA copies in culture media in A549 cells overexpressing GFP or PINK1 then treated with increasing doses of TM for 24h (n = 4; *p<0.01 vs TM 0μg/ml, #p<0.001 vs GFP; two-way ANOVA with multiple comparison). Dot plots represent mean ± SEM. See S1 Fig for details about ER stress markers levels after TM treatment and knock-down / overexpression efficiencies.