| WT |
RHΔku80Δhxg
|
Requested from the Carruthers Lab, not generated in this study |
| Δcrt
|
RHΔku80ΔhxgΔcrt
|
TgCRT was deleted by double-crossover homologous recombination |
| ΔcrtCRT
|
RHΔku80ΔhxgΔcrt::TgCRT-mCherry-3xmyc
|
Ectopic expression of a C-terminally epitope-tagged TgCRT in Δcrt for complementation |
| ΔcrtCRTT369K
|
RHΔku80ΔhxgΔcrt::TgCRTT369K-mCherry-3xmyc
|
Ectopic expression of a C-terminally epitope-tagged TgCRT mutant in Δcrt for complementation. The original threonine at position 369 within TgCRT was changed to lysine by site-directed mutagenesis. |
| ΔcrtΔcpb
|
RHΔku80ΔhxgΔcrtΔcpb
|
The entire TgCPB gene was ablated by CRISPR-Cas9 based genome editing technique |
| ΔcrtΔcpl
|
RHΔku80ΔhxgΔcrtΔcpl
|
The entire TgCPL gene was ablated by CRISPR-Cas9 based genome editing technique |
| ΔcrtΔsub1
|
RHΔku80ΔhxgΔcrtΔsub1
|
The entire TgSUB1 gene was ablated by CRISPR-Cas9 based genome editing technique |
| WT::nLuc
|
RHΔku80Δhxg::nLuc
|
Expressed NanoLuc luciferase in WT parasites |
| Δcrt::nLuc
|
RHΔku80ΔhxgΔcrt::nLuc
|
Expressed NanoLuc luciferase in Δcrt parasites |
| ΔcrtCRT::nLuc
|
RHΔku80ΔhxgΔcrt::TgCRT-mCherry-3xmyc::nLuc
|
Expressed NanoLuc luciferase in ΔcrtCRT parasites |
| TgAMN-3xHA |
RHΔku80ΔhxgTgAMN-3xHA
|
TgAMN gene was endogenously tagged with a 3xHA epitope at its 3'-end by CRISPR-Cas9 based genome editing technique |
| TgSCP-3xmyc |
RHΔku80ΔhxgTgSCP-3xmyc
|
TgSCP gene was endogenously tagged with a 3xmyc epitope at its 3'-end by single-crossover recombination |
| WT::PHL2
|
RHΔku80Δhxg::PHL2
|
Expressed pHluorin2 in the cytoplasm of WT parasites |
| Δcrt::PHL2
|
RHΔku80ΔhxgΔcrt::PHL2
|
Expressed pHluorin2 in the cytoplasm of Δcrt parasites |
| ΔcrtCRT::PHL2
|
RHΔku80ΔhxgΔcrt::TgCRT-mCherry-3xmyc::PHL2
|
Expressed pHluorin2 in the cytoplasm of ΔcrtCRT parasites |
| Δvp1
|
RHΔku80ΔhxgΔvp1
|
Requested from the Moreno Lab, not generated in this study |
| Δvp1VP1
|
RHΔku80ΔhxgΔvp1VP1
|
Requested from the Moreno Lab, not generated in this study |
