Figure 2.

Experimental design for detecting recombination in the extended pseudoautosomal region (PAR) of CAST/EiJ. (A) Marker map near the pseudoautosomal boundary. A schematic representation of the CAST/EiJ extended PAR (green) and canonical PAR (dark gray), with its internal assembly gaps (light gray) and protein-coding genes, is shown in the top panel. Nominal physical positions (in the mm10 assembly) of SNP markers from the 77K and 11K arrays are shown in the bottom panel. CAST/EiJ and canonical PAR boundaries are indicated by red dashed lines. (B) Multiple (PWK/PhJ $\times$ CAST/EiJ)F1 males were bred with FVB/NJ females, and N2 progeny genotyped with commercial SNP arrays. In the absence of recombination, female progeny inherit an maternal FVB/NJ allele and a paternal PWK/PhJ allele at X chromosome markers; nonrecombinant male progeny inherit a maternal FVB/NJ allele and a paternal CAST/EiJ allele. Other combinations require recombination between the X and Y chromosomes.