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. 2019 Jun 6;14(6):e0217495. doi: 10.1371/journal.pone.0217495

Fig 7. Regulation of TNF-α and etanercept (ETA) on the enzymes associated with hormone conversion.

Fig 7

(A-D) The mRNA and protein expressions of CYP19A1 in KGN cells were significantly inhibited after 36 h of persistent treatment with different doses of TNF-α (a,b,c indicate comparison of control with 10, 50, and 100 ng/mL of ETA). However, the influence of TNF-α on CYP17A1 expression could not be determined due to the different expressions of CYP17A1 at different time points compared to that of the control (All groups, *P<0.05 and **P<0.01 by one-way ANOVA; CTL versus TNF-α, #P<0.05 and ##P<0.01 by Dunnett’s multiple comparison test). (E, F) ETA ameliorated the downregulation of CYP19A1 induced by TNF-α. However, ETA-mediated regulation of CYP17A1 was still undetermined (All groups, *P<0.05 and **P<0.01 by one-way ANOVA; CTL versus TNF-α, #P<0.05 and ##P<0.01 by Dunnett’s multiple comparison test; TNF-α versus TNF-α+ETA, &P<0.05 and &&P<0.01 by Dunnett’s multiple comparison test).