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. 2019 Jun 6;14(6):e0217652. doi: 10.1371/journal.pone.0217652

Fig 4. MiR-582-5p directly binds to NOTH1 3’UTR in NCI-H358 cell.

Fig 4

(A) Illustration showed the predicted binding site between miR-582-5p and WT-3'UTR-NOTCH1 or Mut-3'UTR- NOTCH1. (B) Dual luciferase reporter assay was conducted to verify the presumptive binding sites. (C) The mRNA level of NOTCH1 was determined with qPCR assay after NCI-H358 cells were transfected with miR-582-5p mimics. (D) The protein level of NOTCH1, NICD1 and Hes-1 was detected by Western blot assay after NCI-H358 cells were transfected with miR-582-5p mimics. (E) Relative expression of NOTCH1 was quantified with Image J and GraphPad Prism 5.0. Data are expressed as the mean ± SD (n = 3). **P<0.01 and ***P<0.001 versus the scramble group.