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. 2019 Jun 6;14(6):e0217652. doi: 10.1371/journal.pone.0217652

Fig 7. Overexpression of NOTCH1 rescues the miR-582-5p-mediated inhibition in NCI-H358 cell proliferation and invasion.

Fig 7

(A) NCI-H358 cells were co-transfected with miR-582-5p mimics and NOTCH1 plasmids or vectors. And cell proliferation was assessed by CCK-8 assay. (B) Western blot assay was applied to examine EMT markers’ expression. (C) Relative protein expression of E-cadherin and Vimentin is shown. (D) Cell invasion was evaluated with Transwell assay. (E) Relative number of invaded cells is shown. Data are presented as mean ± SD (n = 3). *P<0.05, **P<0.01 and ***P<0.001 versus the scramble group. #P<0.05, ##P<0.01 versus the miR-582-5p mimic group.