Table 1.

Serological analysis of convalescent CHIKV human serum samples

	ELISA		PRNT80 titer
Sample number	CHIKV IgM	CHIKV IgG	CHIKV	MAYV	UNAV
EB3	Neg	Pos	1,280	80	640
EB4	Neg	Pos	160	< 20	< 20
EB5	Neg	Pos	640	< 20	< 20
EB7	Neg	Pos	1,280	40	< 20
EB9	Neg	Pos	< 20	< 20	< 20
EB13	Neg	Pos	1,280	< 20	< 20
EB14	Neg	Pos	2,560	< 20	< 20
EB15	Neg	Pos	640	< 20	160
EB16	Neg	Pos	320	< 20	< 20
EB18	Neg	Pos	320	< 20	40
EB19	Neg	Pos	160	< 20	80
EB20	Neg	Pos	320	< 20	80
EB21	Neg	Pos	320	< 20	< 20
EB22	Neg	Pos	640	< 20	80
EB23	Neg	Pos	1,280	20	80
EB25	Neg	Pos	320	< 20	< 20
EB26	Neg	Pos	160	< 20	40
EB27	Neg	Pos	1,280	40	640
EB28	Neg	Pos	640	40	80
EB29	Neg	Pos	320	< 20	40
EB30	Neg	Pos	1,280	< 20	40
EB31	Neg	Pos	320	80	< 20
EB32	Neg	Pos	2,560	40	80
EB33	Neg	Pos	5,120	< 20	40
EB34	Pos	Pos	1,280	20	< 20
EB35	Neg	Pos	2,560	40	< 20
EB36	Neg	Pos	640	20	20
EB37	Neg	Pos	1,280	< 20	20
EB38	Neg	Pos	640	40	20
EB39	Neg	Pos	2,560	20	40
EB40	Neg	Pos	640	80	< 20
EB41	Neg	Pos	320	20	40
EB42	Neg	Pos	1,280	40	80
EB43	Neg	Pos	640	40	20
EB44	Neg	Pos	1,280	80	40
EB1	Neg	Neg	< 20	< 20	< 20
EB2	Neg	Neg	< 20	< 20	< 20
EB6	Neg	Neg	< 20	< 20	< 20
EB8	Neg	Neg	< 20	< 20	< 20
EB10	Neg	Neg	< 20	< 20	< 20
EB11	Neg	Neg	< 20	< 20	< 20
EB12	Neg	Neg	< 20	< 20	< 20
EB17	Neg	Neg	< 20	< 20	< 20
EB24	Neg	Neg	< 20	< 20	< 20
EB45	Neg	Neg	< 20	< 20	< 20
EB46	Neg	Neg	< 20	< 20	< 20
EB47	Neg	Neg	< 20	< 20	< 20
EB48	Neg	Neg	< 20	< 20	< 20
EB49	Neg	Neg	< 20	< 20	< 20
EB50	Neg	Neg	< 20	< 20	< 20

CHIKV = chikungunya virus; MAYV = Mayaro virus; UNAV = Una virus; PRNT_{80 =} plaque reduction neutralization titer. Samples were tested for anti-CHIKV IgM and IgG antibodies. Neutralizing antibody response to CHIKV, MAYV, and UNAV was determined via PRNT₈₀ assay. Limit of detection was a titer of 1:20 and all negative samples were assigned a titer of < 1:20. All positive samples are indicated in bold and negative samples are indicated in italics.