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. 2019 Mar 13;18(6):1070–1084. doi: 10.1074/mcp.RA119.001416

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© 2019 Liu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Characteristics of fertility of Cmtm4 WT and KO male mice. No variance in testicular weight (A), sperm concentration (B) and serum testosterone level (C) between WT and KO mice was observed. Significantly reduced daily sperm production (D) and sperm motility (E, F, G) and increased abnormal sperm morphology (H, K) were observed in Cmtm4 KO mice compared with WT mice. In vitro fertility analyses showed a decreased percentage of induced acrosome reactions (I, M), the numbers in brackets represent the number of mice examined, and the decreased percentage of oocytes developed to two-cell stages. The numbers in brackets represent the number of WT oocytes used (J, L). Values are shown as mean ± S.D. a, curved back sperm head; b, bent sperm midpiece; WT, wild type; KO, knockout; VSL, straight line velocity; VCL, curvilinear velocity; Lines (E, F, G) indicate mean values, whiskers indicate standard deviation; Data were compared by one-way analysis of variance (ANOVA); *, p < 0.05; **, p < 0.01. Each bar represents 20 μm.