Table II. Root phenotyping for 16 top responsive candidate proteins that exhibit differential expression following auxin treatment. Primary root lengths were measured at 5 and 7 days after germination. For auxin response assays, seedlings (n > 10) were grown for 5 days and then transferred to either control plates (0.5X MS) or 0.5X MS plates supplemented with 1 μm IAA and allowed to grow for two more days. Primary root length and lateral root number were subsequently calculated on treated seedlings. S.E. = standard error. p values were calculated using two-tailed t tests of unequal variance. Significant phenotypes are indicated in bold.
| Genotype | Allele | Protein ID | Root length (mm) +/− S.E. | p value | Primary root phenotype | % Root growth on auxin relative to untreated | p value | No. lateral roots/mm on auxin +/− S.E. | p value | Lateral root phenotype on auxin |
|---|---|---|---|---|---|---|---|---|---|---|
| Col-0 | 19.878 +/− 0.483 | n/a | Wild-type | 78% | 0.23 +/− 0.01 | Wild-type | ||||
| SALK_029319 | gaut10–1 | AT2G20810.1 | 17.821 +/− 0.903 | 0.054 | Short roots | 103% | 0.39 | 0.08 +/− 0.02 | 0.000029 | Reduced lateral roots |
| SALK_014345 | vps35b-1 | AT1G75850.1 | 15.336 +/1 1.387 | 0.013 | Short roots | 126% | 0.45 | 0.22 +/− 0.03 | 0.768 | Wild-type |
| SALK_142260 | bdx-2 | AT4G32460.2 | 19.619 +/− 0.874 | 0.796 | Wild-type | 74% | 0.67 | 0.11 +/− 0.02 | 0.0006 | Reduced lateral roots |
| SALK_007027 | camta2 | AT5G64220.1 | 15.286 +/− 0.658 | 0.000002 | Short roots | 73% | 0.63 | 0.10 +/− 0.02 | 0.00007 | Reduced lateral roots |
| SALK_008979 | gapcp2.2 | AT1G16300.1 | 20.024 +/− 0.928 | 0.890 | Wild-type | 138% | 0.001 | 0.01 +/− 0.008 | 8.20E-07 | No lateral roots |
| SALK_016830 | atrh8 | AT4G00660.2 | 13.714 +/− 1.592 | 0.002 | Short roots | 77% | 0.95 | 0.07 +/− 0.03 | 0.0002 | Reduced lateral roots |
| SALK_127730 | smp2 | AT4G37120.1 | 15.904 +/− 0.755 | 0.0001 | Short roots | 84% | 0.53 | 0.07 +/− 0.02 | 6.54E-06 | Reduced lateral roots |
| SALK_059908 | pme17–2 | AT2G45220.1 | 17.600 +/− 0.716 | 0.012 | Short roots | 79% | 0.84 | 0.07 +/− 0.03 | 0.0001 | Reduced lateral roots |
| SALK_063023 | AT5G09620.1 | 19.602 +/− 0.747 | 0.758 | Wild-type | 70% | 0.51 | 0.05 +/− 0.02 | 5.40E-06 | Reduced lateral roots | |
| SALK_064163 | nadp-me4 | AT1G79750.1 | 18.954 +/− 1.325 | 0.520 | Wild-type | 76% | 0.91 | 0.16 +/− 0.03 | 0.07 | Wild-type |
| SALK_091518 | rhip1–1 | AT4G26410.1 | 20.885 +/− 1.14 | 0.425 | Wild-type | 86% | 0.45 | 0.17 +/− 0.03 | 0.02 | Reduced lateral roots |
| SALK_111575 | clpb3 | AT5G15450.1 | 17.512 +/− 0.814 | 0.017 | Short roots | 87% | 0.49 | 0.13 +/− 0.04 | 0.03 | Reduced lateral roots |
| SALK_129331 | cka2–1 | AT3G50000.1 | 18.683 +/− 0.885 | 0.246 | Wild-type | 68% | 0.19 | 0.13 +/− 0.02 | 0.001 | Reduced lateral roots |
| SALK_145341 | abr | AT3G02480.1 | 17.181 +/− 0.902 | 0.018 | Short roots | 75% | 0.80 | 0.22 +/− 0.03 | 0.181 | Wild-type |
| SALK_151595 | ae3/rpn8a | AT5G05780.1 | 15.824 +/− 0.910 | 0.001 | Short roots | 71% | 0.49 | 0 +/− 0 | 4.90E-08 | No lateral roots |
| SAIL_35_A08 | impl2–3 | AT4G39120.1 | 18.907 +/− 1.206 | 0.461 | Wild-type | 85% | 0.72 | 0.11 +/− 0.02 | 0.0001 | Reduced lateral roots |