| pTrc99A |
Low copy bacterial expression vector with inducible lacI promoter, AmpR
|
Pharmacia Biotech |
| pTrc99A-SseK1 |
sseK1 from S. Typhimurium SL1344 in pTrc99A, AmpR
|
This study |
| pTrc99A-SseK1DxD(229–231)AAA
|
sseK1 from S. Typhimurium SL1344 in pTrc99A, with DxD (229–231) catalytic motif mutated to AAA, AmpR
|
This study |
| pTrc99A-SseK1E255A
|
sseK1 from S. Typhimurium SL1344 in pTrc99A, with Glu255 mutated to Ala, AmpR
|
This study |
| pTrc99A-SseK2 |
sseK2 from S. Typhimurium SL1344 in pTrc99A, AmpR
|
This study |
| pTrc99A-SseK2DxD(239–241)AAA
|
sseK2 from S. Typhimurium SL1344 in pTrc99A with DxD(239–241) catalytic motif mutated to AAA, AmpR
|
This study |
| pTrc99A-SseK2E271A
|
sseK2 from S. Typhimurium SL1344 in pTrc99A, with Glu271 mutated to Ala, AmpR
|
This study |
| pTrc99A-SseK3 |
sseK3 from S. Typhimurium SL1344 in pTrc99A, AmpR
|
This study |
| pTrc99A-SseK3DxD(226–228)AAA
|
sseK3 from S. Typhimurium SL1344 in pTrc99A with DxD(226–228) catalytic motif mutated to AAA, AmpR
|
This study |
| pTrc99A-SseK3E258A
|
sseK3 from S. Typhimurium SL1344 in pTrc99A, with Glu258 mutated to Ala, AmpR
|
This study |
| p3xFlag-Myc-CMV-24 |
Dual tagged N-terminal Met-3xFlag and C-terminal c-myc expression vector, AmpR
|
Sigma-Aldrich |
| pFlag-TRADD |
Human TRADD in p3xFlag-Myc-CMV, AmpR
|
Jürg Tschopp |
| pFlag-TRADDR235A
|
Human TRADD with Arg235 mutated to Ala, in p3xFlag-Myc-CMV, AmpR
|
This study |
| pFlag-TRADDR245A
|
Human TRADD with Arg245 mutated to Ala, in p3xFlag-Myc-CMV, AmpR
|
This study |
| pFlag-TRADDR235A/R245A
|
Human TRADD with Arg235 and Arg245 mutated to Ala, in p3xFlag-Myc-CMV, AmpR
|
This study |
| pFlag-hTRAILR2DD
|
Human TRAILR2 death domain in p3xFlag-Myc-CMV, AmpR
|
This study |
| pFlag-hTNFR1DD
|
Human TNFR1 death domain in p3xFlag-Myc-CMV, AmpR
|
This study |
| pEGFP-C2 |
Expression vector carrying EGFP fused to the N terminus of the partner protein, KanR
|
Clontech |
| pEGFP-C2-SseK1 |
sseK1 from S. Typhimurium SL1344 in pEGFP-C2, KanR
|
This study |
| pEGFP-C2-SseK3 |
sseK3 from S. Typhimurium SL1344 in pEGFP-C2, KanR
|
This study |
| pEGFP-C2-SseK3E258A
|
sseK3 from S. Typhimurium SL1344 in pEGFP-C2, with Glu258 mutated to Ala, KanR
|
This study |
| pET28a |
Bacterial expression vector with T7lac promoter including N-terminal 6 x Histidine tag, KanR
|
Novagen |
| pET28a-hTRAILR2DD
|
Human TRAILR2 death domain in pET28a, KanR
|
This study |
| pGBKT7 |
High copy number yeast expression vector carrying a GAL4 DNA binding domain, KanR (bacterial selection), Trp (selectable marker in yeast) |
Clontech |
| pGBKT7-NleB1 |
nleB1 from EPEC E2348/69 in pGBKT7, KanR,Trp |
This study |
| pGBKT7-SseK3 |
sseK3 from S. Typhimurium SL1344 inpGBKT7, KanR, Trp |
This study |
| pGADT7-AD |
High copy number yeast expression vector carrying a GAL4 activation domain, AmpR (bacterial selection), Leu (selectable marker in yeast) |
Clontech |
| pGADT7-AD-FADDDD
|
Death domain of human FADD in pGADT7-AD, AmpR, Leu |
This study |
| pGADT7-AD-TNFR1DD
|
Death domain of human TNFR1 in pGADT7-AD,AmpR, Leu |
This study |
| pGADT7-AD-hTRAILR2DD
|
Human TRAILR2 death domain in pGADT7-AD, AmpR, Leu |
This study |
| pGEX-4T-1 |
Low copy number N-terminal glutathione-S-transferase fusion vector, AmpR
|
GE Healthcare |
| pGEX-SseK3 |
sseK3 from S. Typhimurium SL1344 in pGEX-4T-1, AmpR
|
This study |
