Table II. Mitochondrial enzyme activities.

Enzyme	Control	Rapamycin	Zinc	Ratio rapa/ctrl	Ratio zinc/ctrl	Shotgun ratio rapa/ctrl	Shotgun ratio zinc/ctrl
Glutaminase	196 ± 35	152 ± 16	121 ± 8	0.77	0.62*	0.48	0.81
l-hydroxyglutarate dehydrogenase	164 ± 30	122 ± 10	114 ± 22	0.74	0.70*	0.82	0.95
d-hydroxyglutarate dehydrogenase	137 ± 23	109 ± 11	102 ± 13	0.80	0.75	0.51	1.01
NAD-isocitrate dehydrogenase	276 ± 11	270 ± 27	219 ± 40	0.98	0.79	ND	ND
Succinate dehydrogenase	82 ± 6	114 ± 8	112 ± 8	1.40**	1.37**	>1.20	>1.13
BPHL (Valacyclovir hydrolase)	842 ± 81	677 ± 72	790 ± 56	0.80*	0.94	0.47	0.60
Citrate synthase	900 ± 97	573 ± 69	614 ± 72	0.64**	0.68**	0.72	0.70
Pyruvate dehydrogenase	188 ± 20	155 ± 14	141 ± 21	0.83*	0.75*	NR	NR

All the activities are expressed in nmole substrate converted/min/mg total protein (mean ± standard deviation). The experiments were carried out on total cell extracts (n = 3, biological replicates). Statistical significance of the results in the heteroscedastic Student T test: * p < 0.05; ** p < 0.01.

ND: not determined, as the enzyme is composed of several subunits.

NR: not relevant, as the pyruvate dehydrogenase activity is controlled by phosphorylation.

ctrl, control; rapa, rapamycin.