Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 30;11:4947–4956. doi: 10.2147/CMAR.S202268

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Gong et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Hypoxia exposure affects the activation of LX2 cells and increases cell apoptosis and GAPDH nuclear translocation. (A and B) Cell apoptosis was detected using annexin V-FITC/PI double staining and flow cytometry (A), and bar graphs (B) show the effect of hypoxia on apoptosis. (C) Cell viability was measured using the MTT assay after hypoxia. (D and E) Western blots showing the effects of hypoxia on the activation of LX2 cells (D) and the accumulation of nuclear GAPDH (E). Untreated samples served as controls.

Note: Data are presented as the mean ± SEM. n.s., not significant, *p<0.05, **p<0.01, and ***p<0.001, n=3.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; α-SMA, α-smooth muscle actin; HIF-1α, hypoxia-inducible factor 1α.