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. 2019 Feb 18;160(6):1281–1296. doi: 10.1097/j.pain.0000000000001523

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Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 3. — Application of PDGF-BB leads to inhibition of I_M in DRG neurons. (A) Voltage-clamp perforated patch recordings from DRG neurons showing families of I_M evoked by a series of 1-second, 5-mV hyperpolarizing voltage steps from a holding potential of −20 mV (shown in inset). The 3 subpanels show the current responses before (left), after focal application of 125 ng/mL of PDGF-BB (middle), and after bath application of 10 μM of XE991 on top of focal application of PDGF-BB (right). The dotted line indicates zero current level. The current response obtained by stepping to −45 mV is shown at the bottom of each subpanel. The I_M relaxation was fitted with a biexponential line (red), which was extrapolated to the beginning of the voltage step. (B) Subtracted trace of I_M evoked by stepping to −45 mV before the application of PDGF-BB minus I_M trace evoked by stepping to −45 mV after PDGF. (C) Left, bar graph depicting mean ± SEM of peak I_M amplitude (measured by stepping to −45 mV), before (gray), 10 minutes after the application of PDGF-BB (red), and 10 minutes after the treatment with XE991 on top of PDGF-BB (orange). **P < 0.01, ns, not significant, RM one-way ANOVA with post hoc Bonferroni test, n = 7 neurons. Right, same as left but showing that a 10-minute application of vehicle (light gray) has no effect on I_M amplitude, ns, not significant, *P < 0.05, RM one-way ANOVA with post hoc Bonferroni test n = 5 neurons. (D) Peak I_M amplitude (measured by stepping to −45 mV, mean ± SEM), plotted vs time of application of PDGF-BB. Platelet-derived growth factor-BB application is indicated by the arrow (time “0”). One-way ANOVA with post hoc Bonferroni test, n = 13 neurons for 6 minutes, n = 11 neurons for 9 minutes. In 7 out of 9 neurons, ∼15 minutes washout of PDGF-BB led to full recovery of I_M. ns, not significant; **P < 0.01. ANOVA, analysis of variance; DRG, dorsal root ganglion; PDGF, platelet-derived growth factor; RM, repeated-measures.