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. 2019 Mar 28;139(22):2570–2584. doi: 10.1161/CIRCULATIONAHA.118.036099

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© 2019 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 5. — VEGF stimulation induces HB-EGF and ADAM12 mRNA expression and Nrg1 release in endothelial cells. A through D, Human cardiac arterial (HCEAC), cardiac microvascular (HCMEC), dermal microvascular (HDMEC), and umbilical vein endothelial cells (HUVEC) were stimulated with vascular endothelial growth factor (VEGF; 100 ng/mL) for 4 hours, and mRNA expression was analyzed. E, Nrg1 levels in conditioned medium from HCMECs stimulated with VEGF. F, Phosphorylation of Akt in cardiomyocytes (CMC) treated with conditioned medium (CM) from HCMECs stimulated with VEGF. Note that Akt activation is blocked with the anti-Nrg1 antibody. HB-EGF indicates heparin-binding epidermal growth factor–like growth factor; Ig, immunoglobulin; and Nrg1, neuregulin 1. Student t test (A through D) and 1-way ANOVA (E); **P<0.01, ***P<0.001 (N=3 per group).