Figure 4.

Printing therapeutics in 3D. (a) The high surface area and charged characteristics of nanosilicates are able to sequester protein therapeutics within 3D printing structure. The degradation of printed network result in release of therapeutics. (b) The release of fluorescently labeled protein from 3D printed structure was monitored over 28 days in PEGDTT (10% wt/vol)/nSi (4% wt/vol) hydrogels (no PEG-DA in formulation). Printed structure dimensions ~ 10 mm diameter by 15 mm height (significantly larger mass than used in degradation studies) (c) Sustained release of sequester therapeutics was observed during initial time period, while complete protein was released within 28 days in PEGDTT (10% wt/vol)/nSi (4% wt/vol) hydrogels (n=3, *p<0.05, one-way ANOVA with Tukey post-hoc testing).