Skip to main content
. Author manuscript; available in PMC: 2020 May 31.
Published in final edited form as: J Mol Biol. 2019 Apr 30;431(12):2354–2368. doi: 10.1016/j.jmb.2019.04.034

Fig 5. Comparison of replication capacity and infectious virus yield of H77S.3 versus N.2 RNAs with the Q80K substitution in the NS3–4A protease.

Fig 5.

(A) The effect of NS3-Q80K on the RNA replication capacity was measured by GLuc assay in the H77S.3/GLuc2A (left, upper panel) or the N.2/GLuc2A genetic background (left, lower panel). Results are normalized to GLuc activity at 6 hrs post transfection, and represent the mean ± standard deviation of triplicate samples. (B) The effect of NS3-Q80K on the infectious virus production from the H77S.3 (right, upper panel) or the N.2 background (right, lower panel) was measured by a focus forming unit (FFU) assay from cell culture supernatant fluids collected every 24 hr post transfection. Data represent the mean ± standard deviation from at least 3 independent experiments.