Table 1.
Hemodynamics, heart weight, and pulmonary vascular analysis in smLRP1−/− and control mice, either untreated or treated with the PPARγ agonist pioglitazone.
| Control (C) |
Control + Pio (CP) |
smLRP1−/− (L) |
smLRP1−/− + Pio (LP) |
p | n | |
|---|---|---|---|---|---|---|
| 10- to 12-week-old | ||||||
| Body weight (g) | 27.5 ± 2.3 | 28.0 ± 2.9 | ||||
| Hemodynamics | ||||||
| HR (bpm) | 328 ± 17 | 337 ± 27 | 3 – 5 | |||
| RVSP (mmHg) | 23.6 ± 0.7 | 31.1 ± 0.8** | 3 – 5 | |||
| RVEDP (mmHg) | 2.0 ± 0.4 | 2.8 ± 1.2 | ** C vs L | 3 – 5 | ||
| 15- to 17-week-old | ||||||
| Body weight (g) | 24.4 ± 1.1 | 26.3 ± 1.4 | 27.7 ± 1.5 | 26.0 ± 0.8 | ||
| Hemodynamics | ||||||
| HR (bpm) | 291 ± 14 | 283 ± 14 | 286 ± 14 | 270 ± 11 | 9 – 11 | |
| RVSP (mmHg) | 23.0 ± 0.7 | 21.5 ± 0.5 | 33.4 ± 2.4** | 22.6 ± 0.6** | ** C vs L, ** CP vs L, ** L vs LP | 9 – 11 |
| RVEDP (mmHg) | 3.1 ± 0.5 | 3.5 ± 0.6 | 6.5 ± 0.5** | 3.4 ± 0.5** | ** C vs L, ** CP vs L, ** L vs LP | 9 – 11 |
| LVSP (mmHg) | 100.2 ± 1.9 | 98.0 ± 3.1 | 108.5 ± 3.4 | 103.8 ± 5.1 | 6 – 11 | |
| LVEDP (mmHg) | 9.8 ± 1.4 | 9.9 ± 1.5 | 13.7 ± 3.1 | 11.3 ± 1.7 | 6 – 11 | |
| SBP (mmHg) | 100.9 ± 1.7 | 98.5 ± 3.4 | 98.6 ± 3.4 | 99.9 ± 3.8 | 7 – 13 | |
| DBP (mmHg) | 67.3 ± 1.4 | 63.9 ± 2.9 | 55.9 ± 2.4** | 59.0 ± 1.2* | ** C vs L, * C vs LP | 7 – 13 |
| MAP (mmHg) | 78.5 ± 1.4 | 75.4 ± 3.1 | 70.2 ± 2.7 | 72.7 ± 2.0 | 7 – 13 | |
| Cardiac Mass | ||||||
| RV/BW (mg/g) | 0.60 ± 0.03 | 0.73 ± 0.04 | 0.93 ± 0.10** | 0.71 ± 0.07 | ** C vs L | 11 – 14 |
| RV/(LV+S) | 0.22 ± 0.01 | 0.26 ± 0.01 | 0.35 ± 0.02** | 0.24 ± 0.01** | ** C vs L, ** CP vs L, ** L vs LP | 11 – 14 |
| LV+S/BW (mg/g) | 2.70 ± 0.07 | 2.83 ± 0.14 | 2.67 ± 0.30 | 2.97 ± 0.19 | 11 – 14 |
At 10–12 week-old, smLRP1−/− and control mice received either regular chow or were orally treated with pioglitazone (20 mg/kg/day) incorporated into the chow for 5 weeks. Data were generated at the age of 10–12 and 15–17 weeks, and are shown as mean ± SEM. Statistically significant differences between groups are indicated with stars (*p<0.05; **p<0.01; student’s t-test or ANOVA, Bonferroni posthoc test). HR, heart rate; bpm, beats per minute; RVSP, right ventricle systolic pressure; RVEDP, right ventricle end diastolic pressure; LVSP, left ventricle systolic pressure; LVEDP, left ventricle end diastolic pressure; SBP, systolic blood pressure; DBP, diastolic blood pressure; MAP, mean arterial pressure, calculated as MAP = (SBP + 2 × DBP): 3; RV/BW, right ventricle / body weight; RV/(LV+S), right ventricle / (left ventricle + septum); (LV+S)/BW, (left ventricle + septum) / body weight.