Figure 3. A Shared Neuronal Population is Activated by Different Anesthetics.
(A) Schematic diagram of CANE technology.
(B) Viral construct and injection site in FosTVA mice.
(C) Illustration of the SON and paraSON. paraSON is defined as a region within 500 μm radius circle with the up-corner of the optic chiasm/tract as the center, extending from anterior to posterior hypothalamus.
(D) Left panels, representative images of CANE-captured isoflurane-activated neurons (red) and Fos+ neurons (green) induced by re-exposure to either isoflurane again, or to Propofol, Ketamine (plus xylazine), or dexmedetomidine (Dex). Right panels, pie charts showing the percentage of initial CANE-captured isoflurane-activated neurons that are re-activated (Fos+) by different anesthetics in SON as well as in paraSON. Neurons were from 7–30 slices from 2–4 mice for each condition.
(E) Whole-cell patch-clamp recording of CANE-captured isoflurane-activated neurons in acute brain slices following treatments of different classes of anesthetics. Top, representative membrane potential changes after the application of drugs; bottom, statistical summary for all recorded neurons. n = 11 neurons for isoflurane; n = 32 for Propofol; n = 25 for Ketamine; n = 27 for Dex. Wilcoxon signed-rank tests for all drugs.
Data are presented as mean ± s.e.m. **P < 0.01, ***P < 0.001.
See also Figure S3