Figure 4. Tuning of Mascot score to more or less permissive thresholds did not improve iTRAQ quantitation performance.

Linear regression plus Pearson correlation coefficient test (A and C) and Bland–Altman comparison (B and D) to assess the correlation and agreement between RSPI and Western blot ratios in five independent experiments (biological replicates) after adjustment to more (A and B) or less (C and D) permissive Mascot score.